Coding

Part:BBa_K4769218:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


Porphyran utilisation locus fragment 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1097
    Illegal XbaI site found at 3641
    Illegal XbaI site found at 4611
    Illegal SpeI site found at 1716
    Illegal PstI site found at 5057
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1097
    Illegal SpeI site found at 1716
    Illegal PstI site found at 5057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1097
    Illegal BamHI site found at 6600
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1097
    Illegal XbaI site found at 3641
    Illegal XbaI site found at 4611
    Illegal SpeI site found at 1716
    Illegal PstI site found at 5057
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1097
    Illegal XbaI site found at 3641
    Illegal XbaI site found at 4611
    Illegal SpeI site found at 1716
    Illegal PstI site found at 5057
    Illegal NgoMIV site found at 1974
    Illegal AgeI site found at 977
    Illegal AgeI site found at 4756
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 6070


Design Notes

N/A


Source

P. plebeius genome

Primers for cloning: FW: gctcatatttaaaaataccactctatatagatagaaatacc (+ desired overhangs) RV: ttagaaattaagacttaaaccaaatctgaatgtac (+ desired overhangs)

References